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Relative mRNA expression of <t>ENO1</t> normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisk (*) indicates statistically significant differences between healthy and ketotic cows at the same time point ( p < 0.05). Error bars represent standard error of the mean (SEM).
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Relative mRNA expression of <t>ENO1</t> normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisk (*) indicates statistically significant differences between healthy and ketotic cows at the same time point ( p < 0.05). Error bars represent standard error of the mean (SEM).
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Relative mRNA expression of <t>ENO1</t> normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisk (*) indicates statistically significant differences between healthy and ketotic cows at the same time point ( p < 0.05). Error bars represent standard error of the mean (SEM).
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Relative mRNA expression of ENO1 normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisk (*) indicates statistically significant differences between healthy and ketotic cows at the same time point ( p < 0.05). Error bars represent standard error of the mean (SEM).

Journal: International Journal of Molecular Sciences

Article Title: Differential Impact of Zearalenone on Hepatic Glucose and Lipid Metabolism in Healthy and Ketotic Dairy Cows: An In Vitro Study

doi: 10.3390/ijms26146827

Figure Lengend Snippet: Relative mRNA expression of ENO1 normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisk (*) indicates statistically significant differences between healthy and ketotic cows at the same time point ( p < 0.05). Error bars represent standard error of the mean (SEM).

Article Snippet: The resulting pre-amplified cDNA preparations were analyzed by real-time PCR in a StepOnePlus Real-time PCR System (Applied Biosystems) using TaqMan Gene Expression Assays and TaqMan Gene Expression Master Mix containing ROX (Applied Biosystems, Foster City, CA, USA) for the following genes: ENO1 (Bt03230937_m1), PDHB (Bt03269405_m1), PGAM1 (Bt03225424_g1), PGK1 (Bt03225857_m1), TPI1 (Bt03224990_m1), ACOX1 (Bt03244689_m1), ACAA1 (Bt03221026_m1), ACACA (Bt03213371_m1), FADS2 (Bt03256252_m1), FASN (Bt03210481_m1), HMGCR (Bt03258816_m1) and SC4MOL (Bt03258188_m1).

Techniques: Expressing, Derivative Assay, Control